Nkọwa ngwaahịa

Mkpado ngwaahịa

Ngwa ngwa ngwa KI Mouse nhazi

ngwa ngwa-ngwa ngwa-ki-mouse-nhazi-ngwaahịa

Knock-in (Knock-in, KI) bụ usoro na-eji nchikota nke mkpụrụ ndụ ihe nketa na-ebufe mkpụrụ ndụ na-arụ ọrụ exogenous n'ime usoro homologous n'ime cell na genome, wee nweta nkwupụta nke ọma na sel mgbe ejikọtaghachi mkpụrụ ndụ ihe nketa.

Ọ na-ewe ọnwa 5-6 iji nweta ụmụ oke heterozygous Neo-ehichapụ na ngụkọta nke ọnwa 10-12 iji wuo ụdị òké KI homozygous nwere ọnụego ọganiihu dị ala site na iji usoro ọdịnala, n'ihi na usoro ndị a nwere obere chimera positivity ma chọọ mating na òké. nweta ụmụ oke heterozygous Neo-ehichapụ, wee mụọ ụmụ oke heterozygous amụrụ maka ọgbọ 2-3 iji nweta ụmụ oke KI homozygous.

Ọgbọ ọhụrụ nke teknụzụ nkwadebe ngwa ngwa ngwa ngwa

TurboMice™

Anyị nwere ike ịnye gị ụdị òké KI homozygous ngwa ngwa site na itinye teknụzụ TurboMice ™ iji kwalite ọnụego ọganiihu.

Dabere na ezigbo mmemme ndezi mkpụrụ ndụ ihe nketa nke ndị ọkà mmụta sayensị anyị, anyị nwere ike mezue nyocha nke mkpụrụ ndụ sel embrayo edeziri n'ime ụbọchị 3-5, wee wuo embrayo tetraploid.Mgbe imechara nwa nwa, enwere ike nweta ụmụ oke homozygous nke mmadụ n'ime ọnwa 2-4, nke nwere ike ịchekwa ọnwa 7-8 maka ndị ahịa.

Ọdịnaya ọrụ

Ọrụ Mba. Ndekọ teknụzụ Ọdịnaya nnyefe okirikiri nnyefe
MC002-1 ogologo otu mkpụrụ ndụ ihe nketa <5kb 3-9 KI ụmụ oke oke homozygous Ọnwa 2-4
MC002-2 ogologo otu mkpụrụ ndụ ihe nketa <5kb 10-19 KI ụmụ oke nwoke homozygous Ọnwa 2-4
MC002-3 ogologo otu mkpụrụ ndụ ihe nketa <5kb 20 KI ụmụ oke oke homozygous Ọnwa 3-5
MC002-4 ogologo nke otu mkpụrụ ndụ ihe nketa bụ 5kb-10kb 3-9 KI ụmụ oke oke homozygous Ọnwa 3-4
MC002-5 ogologo nke otu mkpụrụ ndụ ihe nketa bụ 5kb-10kb 10-19 KI ụmụ oke nwoke homozygous Ọnwa 3-4
MC002-6 ogologo nke otu mkpụrụ ndụ ihe nketa bụ 5kb-10kb 20 KI ụmụ oke oke homozygous Ọnwa 3-5

Kpọtụrụ anyị

Biko kpọtụrụ anyị na:

1) Biko budata ma dejupụta fọm dị n'okpuru ebe a《Mpempe arịrịọ nhota okwu》, ma ziga ya site na email naMingCelerOversea@mingceler.com;

2) Te: +86 181 3873 9432;

3) LinkedIn: https://www.linkedin.com/company/mingceler/

Mpempe arịrịọ nhota okwu.docx

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